It has been known for years that germ cells are

2018-10-20

It has been known for years that germ Bindarit are highly sensitive to genotoxic reagents. This high level of apoptosis may provide an important mechanism to prevent abnormal germ cells from transmitting genetic information to the next generation. In this context, our results suggest that Trp53 app

br Results br Discussion In this study

2018-10-20

Results Discussion In this study, we integrated transcriptome and proteome data and characterized the inventory of HSPCs and their immediate myeloid committed progeny (LS−K) (Figure S6). With almost 20,000 identified expressed genes including more than 3,000 significantly regulated ones, these

microRNAs miRNAs are small noncoding RNAs of nucleotides tha

2018-10-20

microRNAs (miRNAs) are small noncoding RNAs of 22 nucleotides that control gene expression chiefly by translational repression (Lee et al., 2004). miRNA expression is spatially and temporally regulated during development, and they can participate in the regulation of pluripotent stem cell lineage co

The derivation of human induced

2018-10-20

The derivation of human induced pluripotent stem pramipexole dihydrochloride (hiPSCs) from fibroblasts (Takahashi et al., 2007) offers new opportunities to study XCI in human cells. For mouse fibroblasts, it has been shown that the Xi becomes reactivated during the reprogramming process, followed by

br Future perspectives Human fetal tissue research and non h

2018-10-20

Future perspectives Human fetal tissue research and non human primate macaque models have been essential to the establishment of human PGCLC differentiation models to study mechanisms that contribute to early PGC allocation in the embryo. Together, these approaches have revealed that humans have

Human mesenchymal stem cells are adult multipotent stromal c

2018-10-20

Human mesenchymal stem OF-1 are adult multipotent stromal cells which differentiate into multiple cell types (Bianco et al., 2013; Castro-Manrreza and Montesinos, 2015). While in terms with the expeditious advances in the field of mesenchymal stem cell (MSC) research, the ambiguity of its terminolo

Most remarkably we identified Nppb Bnp

2018-10-20

Most remarkably, we identified Nppb (Bnp), a well-studied marker in cardiovascular disease, as a novel putative Shox2 target gene. This gene was analyzed as working myocard marker and turned out to be significantly upregulated in Shox2 SAN-like plk1 inhibitor and in right atrial tissue of Shox2-KO e

br Acknowledgements We extend our sincere thanks to Cindy Ch

2018-10-20

Acknowledgements We extend our sincere thanks to Cindy Cheung for her help with the immunostaining, Yuhuan Wang for her help with daily cell culture, and Atena Zahedi for growing the GFP-RAC activated plasmid. The pcDNA3-EGFP-Rac1-Q61L plasmid (Plasmid # 12981) was created by Gary Bokoch and purc

Like any other method karyotyping cannot prove the absence o

2018-10-20

Like any other method, karyotyping cannot prove the absence of variant cells. Nonetheless, mosaicism can be excluded to a certain degree by analyzing an appropriate number of metaphases (Hook, 1977). The exact number of metaphases scored may depend on the ultimate application of cells. For example,

br Experimental Procedures br Introduction Cell based advanc

2018-10-20

Experimental Procedures Introduction Cell-based advanced therapy medicinal products (ATMPs) have the potential to transform our health care systems, especially for patients with failing intrinsic tissue regeneration such as complex and large bone defects in compromised biological conditions (E

Hello world!

2018-07-29