• Differentiation of NPCs was induced by

  2018-11-08

  

  Differentiation of NPCs was induced by replacing the culture medium by a medium without growth factors and containing 1% FBS. Under this condition, NPCs generated astrocytes and neurons. Astrocyte was the most abundant cell type, present in a proportion of 89.6% (±1.3%). The presence of taurine in t

• br Materials and methods br

  2018-11-08

  

  Materials and methods Author disclosure statement Acknowledgments We thank Muscle Tissue Culture Collection (MTCC) for providing the sample. The Muscle Tissue Culture Collection is part of the German network on muscular dystrophies (MD-NET, service structure S1, 01GM0601) and the German net

• br Resource table br Resource details An induced

  2018-11-08

  

  Resource table Resource details An induced pluripotent stem cell (iPSC) line had been generated from human skin fibroblasts of a male, symptomatic 33-year-old spinocerebellar type 2 (SCA2) patient (anonymized as H271) using episomal vectors carrying transcripts for human OCT4, SOX2, KLF4, L-MY

• Fortunately a new class of RNA guided

  2018-11-08

  

  Fortunately, a new class of RNA-guided nucleases has been developed from microbial adaptive immune systems (i.e. clustered regularly interspaced short palindromic repeat-CRISPR- and CRISPR associated Cas9-CRISPR/Cas9-systems) (Cong et al., 2013; Mali et al., 2013a). This system provides a very simpl

• Based on the differentiation and siRNA

  2018-11-08

  

  Based on the differentiation and siRNA studies reported here, the mechanistic regulation of FOXM1 towards pluripotency control and differentiation might differ between mouse and human ES cells. The discrepancy can be attributed to the different developmental status of human versus mouse ES cells (Ni

• In our current study IGFBP levels were approximately

  2018-11-08

  

  In our current study IGFBP-2 levels were approximately 10-fold higher than those of IGFBP-3 suggesting that the large induction of IGFBP-2 may play a more import role in the differentiation of DPCs than the decrease seen in IGFBP-3 concentrations. Although an extensive literature describes both IGF-

• An important question that remains

  2018-11-08

  

  An important question that remains an area of ongoing work is to determine whether the sustained presence of Tregs after hNPC rejection continues to dampen neuroinflammation long term. We believe this is possible because hNPCs had already disappeared from the spinal cord at the time we detected incr

• Q-VD-Oph cost Introduction Cell to cell competition among ma

  2018-11-08

  

  Introduction Cell-to-cell competition among male germline stem cells has been proposed to account for the higher frequency of some disorders among children of older fathers, a correlation referred to as the paternal age effect (PAE) (Goriely and Wilkie, 2012). In addition to compelling observations

• A variety of suitable murine xenograft models are currently

  2018-11-08

  

  A variety of suitable murine xenograft models are currently used in hematopoietic research, yet these animals require conditioning prior to experimentation. Given the increasingly restricted access to radiation sources, NBSGW mice are broadly enabling, allowing laboratories without access to radiati

• br Results br Discussion Two different

  2018-11-08

  

  Results Discussion Two different lineages of BAs have been known. Classical BAs are derived from Myf-5 expressing common progenitors for myoblasts and BAs (Seale et al., 2008). The myoblastic precursors originate from the mesenchymal stem pp1 (MSCs). PRDM16 is crucially involved in the differ

• br Results br Discussion Our studies highlight

  2018-11-08

  

  Results Discussion Our studies highlight the potential clinical significance of the iC9 safeguard system. Recent studies introducing suicide gene-based safeguard systems into mouse or rhesus iPSCs report that teratoma formation was significantly delayed or inhibited only when treatment started

• br Experimental Procedures Detailed information on the

  2018-11-08

  

  Experimental Procedures Detailed information on the experimental procedures can be found in the Supplemental Information File. Author Contributions Introduction Although cell-fate decisions are fairly stable in vivo, somatic inhibitor of apoptosis proteins can be reprogrammed back into plu

• br Results br Discussion This study describes

  2018-11-07

  

  Results Discussion This study describes a simplified protocol for the efficient generation of hiNSCs via direct reprogramming. hiNSC lines grow as colonies on MEF feeder layers. Like hESCs, hiNSCs stain positive for pluripotent transcription factors OCT4, SOX2, and NANOG, but do not express ce

• Introduction A transparent cornea is

  2018-11-07

  

  Introduction A transparent cornea is crucial for optimal vision. Ocular trauma, a leading cause of loss of corneal transparency, accounts for approximately 25 million cases of blindness annually (Resnikoff et al., 2008; Whitcher et al., 2001). During ocular injury, inflammation-induced transforming

• The specific sources of data for each

  2018-11-07

  

  The specific sources of data for each section of the paper are described in Appendix A. Results Discussion From the above observations we draw a series of ten inferences, presented roughly in the order of those least to most speculative; since we have offered no identification strategy in the

15641 records 1027/1043 page Previous Next First page 上5页 10261027102810291030 下5页 Last page